Agilent Bio SEC
Agilent Bio SEC HPLC Columns from Agilent Technologies
Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle. Agilent Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.
Agilent Bio SEC-5 HPLC columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Agilent Bio SEC-5 HPLC columns are available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å.
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- Features
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- Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
- Sharper peaks, higher resolution, and better protein recovery
- Faster separations than large-particle SEC columns
- Compatibility with most aqueous buffers
- Excellent stability in high-salt and low-salt conditions
- Specifications
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Agilent Bio SEC-3 Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate 100Å 3 µm 100-100,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 150Å 3 µm 500-150,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 300Å 3 µm 5,000-1,250,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) Agilent Bio SEC-5 Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate 100Å 5 µm 100-100,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 150Å 5 µm 500-150,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 300Å 5 µm 5,000-1,250,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 500Å 5 µm 15,000-5,000,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 1000Å 5 µm 50,000-7,500,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 2000Å 5 µm >10,000,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id)